Application

Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.

Protease is used to degrade and remove protein during nucleic acid purification and other molecular biological methods.

Preparation Note

Collected from culture broth of S. griseus and purified by successive column procedures.

Specificity

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole （181 μg） of tyrosine per min at pH 7.5 at 37 °C （color by Folin-Ciocalteu reagent）.

Physical properties

Compley inactivated by heating above 80 °C for 15-20 minutes.